Analysing caffeine in coffee with HPLC


AM Session: 9:30am – 1:30pm
Session timings may be adjusted slightly to suit school calendars between the hours of 9:00 – 4:30.

Curriculum Areas

VCE Chemistry

Students will examine the chemical structure of caffeine molecules and use the HPLC to generate a calibration curve using caffeine standards. Students will then prepare samples of coffee containing unknown amounts of caffeine and use the HPLC to determine the amount of caffeine in each sample. They will then compare the results to the recommended daily amount from the Mayo Clinic.