OUTCOMES
› The use of enzymes to manipulate DNA
› Amplification of DNA using PCR and the use of gel electrophoresis in sorting DNA fragments
› The use of recombinant plasmids as vectors to transform bacterial cells
1 day 9:30 – 2:30
Max Number of Students22
Students explore the essential technique of genetic transformation, enabling protein production. In this session, students will use green fluorescent protein (GFP) to transform E. coli bacteria with a plasmid, allowing the bacteria to express new genetic information. They will perform a DNA digest and confirm the creation of recombinant plasmids through gel electrophoresis. This practical experience supports Unit 3, Outcome 1 of the VCE Biology curriculum, focusing on nucleic acids, proteins, and DNA manipulation techniques.
Students will engage in activities like bacterial transformation with the pGlo plasmid, DNA digestion, and
gel electrophoresis. These hands-on tasks can lead to School Assessed Coursework (SACs) such as bioethical issue analysis, data evaluation, and comparisons of biological concepts. The workshop covers key curriculum points, including enzyme use in DNA manipulation, DNA amplification via PCR, and using recombinant plasmids as vectors. This program gives students a solid understanding of genetic transformation and its biotechnological applications.
› The use of enzymes to manipulate DNA
› Amplification of DNA using PCR and the use of gel electrophoresis in sorting DNA fragments
› The use of recombinant plasmids as vectors to transform bacterial cells
