VCE Chemistry – Analysing Caffeine

In this engaging program, students will delve into the chemical structure and biological effects of caffeine. Using state-of-the-art High- Performance Liquid Chromatography (HPLC) equipment, they will analyse the caffeine content in various drinks. Students will begin by using micropipettes to prepare caffeine standards and run these through the HPLC. This hands-on approach not only enhances their understanding of caffeine’s chemical structure but also teaches them essential laboratory techniques.

Students will gain practical experience in using HPLC instrument, interpreting chromatograms, and determining caffeine concentrations in samples such as instant and decaf coffee. They will learn the principles of chromatography, understand how an HPLC instrument functions, and use Excel to construct calibration curves and perform calculations. By the end of the program, students will understand the importance of accuracy versus precision in scientific measurements and explore the applications of chromatography in various industries, equipping them with a robust skill set for future scientific endeavors.